METHOD FOR PRODUCING PROTEINS HAVING EFFECTIVITY AGAINST PLANT ILLNESSES CAUSED BY FUNGI AND ANOTHER ONE FOR PRODUCING PLANT SORTS HAVING LOW SENSIBILITY TO INFECTIONS BY FUNGI

摘要

New methods are provided for the identification and purification of proteins from plants with an inhibitory effect on pathogens and/or pests, such as insects, nematodes, fungi, and bacteria, using a combination of non-denaturing protein fractionation techniques on leaf-extracts of plants which were treated to develop induced resistance and an antipathogenic assay. Also provided are antifungal proteins obtained by the said method, recombinant nucleotide sequences encoding the said proteins, as well as plants with reduced susceptibility to fungi due to production and desired targetting of the said proteins in the plant.