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FREMGANGSMAATE FOR FREMSTILLING AV EN YARROWIA LIPOLYTICA-TRANSFORMANT SOM IKKE PRODUSERER ALKALISK PROTEASE, SAMT FREMGANGSMAATE FOR PAAVISNING AV Y. LIPOLYTICA-TRANSFORMANTER
FREMGANGSMAATE FOR FREMSTILLING AV EN YARROWIA LIPOLYTICA-TRANSFORMANT SOM IKKE PRODUSERER ALKALISK PROTEASE, SAMT FREMGANGSMAATE FOR PAAVISNING AV Y. LIPOLYTICA-TRANSFORMANTER
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机译:制作不产生碱性蛋白酶的横纹肌萎缩性脂肪变性变压器的程序,以及检测脂解性黄萎病脂质体的程序
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摘要
Sequencing of the XPR2 and LEU2 genes of Yarrowia lipolytica, recombinant Yarrowia lipolytica cloning vehicles comprising heterologous DNA coding for the expression of mammalian protein and other polypeptides, including plasmids suited for transformation of Y. lipolytica hosts and incorporating a regulon homologous to the host in its untransformed state, and secretion signals for the heterologous gene; integrative expression vectors using the XPR 2 gene promoter, alkaline protease pre-proregion and XPR2 terminator region and those having the LEU2 promoter and alkaline protease secretory signal sequences capable, in a transformed Y. lipolytica cell culture, of expressing and secreting a heterologous protein outside the cell; Y. lipolytica transformants comprising said vectors and plasmids; methods for preparing vectors to direct secretion of specified heterologous proteins coded for by genes, cDNA or synthetic DNA in Y. lipolytica in their mature, functional state.
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