ENZYMATIC METHOD OF DETERMINING Ÿ-LACTAM ANTIBIOTICS IN BIOLOGICAL FLUIDS

摘要

It entails the following stages: 1) incubation of the biological fluid with the soluble D-alanyl-D-alanine carboxypeptidase produced by the strain Actinomadura R39; 2) incubation of the mixture obtained with a solution of substrate, which is a thioester of formula …IMAGE… in the presence of an amino acid of D configuration or glycine; 3) determination of the quantity of 2-mercaptoalkanoic acid of formula …IMAGE… formed in stage 2); 4) comparison of the determination of stage 3) with a standard. …??In these formulae: … …  R1 = benzoyl, phenylacetyl or Nalpha-acetyl-Llysyl, …  R2 = glycyl or D-alanyl, and …  R3 = H or CH3. … …??The invention also relates to a test kit comprising the reagents to be employed for implementation of the method.