METHOD FOR PREPARING A CULTURAL ENVIRONMENT OF MAMIFERO CELLS, FREE OF BASIC MEDIUM, HYDROLYSED YEAST, DEXTRAN OR ALBUMINE, INSULINATRANSFERRIN, FRUTOSE FERRICA. CITRATE AND FERROUS SULFATE AND A COMPOUND OF FATTY ACID

摘要

The invention provides serum-free media for the culture of mammalian cells comprising a synthetic basal medium designed for mammalian cell culture; about 0.1 to about 10 grams per liter hydrolyzed yeast; about 0.1 to about 5 grams per liter of dextran or albumin; about 2 to about 20 milligrams per liter insulin; 0 to about 100 milligrams per liter of a compound selected from the group consisting of transferrin, ferric fructose, ferrous citrate and ferrous sulfate; and a fatty acid component consisting of oleic acid, linoleic acid and linolenic acid in a ratio of about 0.6: 1: 0.14 milligrams of fatty acid per liter.