INHIBITION OF HUMAN IMMUNODEFICIENCY VIRUS BY AN ADENO-ASSOCIATED VIRUS GENE FOR HUMAN CELLS

摘要

A recombinant plasmid is disclosed according to the invention which comprises a transcription promoter inducible by gene products expressed by a human lentivirus and a gene which is non-homologous to that human lentivirus. The non-homologous gene encodes a gene product which inhibits lentiviral growth and lentiviral protein production by interfering with the trans-acting gene regulation system of the lentivirus. The expression of the non-homologous gene is further increased by the above-mentioned transcription promoter. The invention further comprises a method of inhibiting lentiviral growth and lentiviral protein production in mammals by transfecting cells of mammals infected with the lentivirus with the above-mentioned recombinant plasmid. The invention further comprises a method of inhibiting lentiviral growth and production by administering to mammals infected with the lentivirus the Rep protein encoded by the above-mentioned non-homologous gene in a pharmaceutically acceptable carrier.