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Method for the detection and quantitative determination of nitrosomonas strains in waste water or soil

机译:废水或土壤中亚硝化菌菌株的检测和定量测定方法

摘要

The detection method for the determination of Nitrosomonas strains in waste water and soil makes use of a gene probe which, by reason of its complementary sequences, hybridises only with parts of the genome of Nitrosomonas strains from samples of waste water or soil and does not give a positive hybridisation signal with parts of the genome of other bacteria and entails the hybridised nucleic acid being determined quantitatively with the aid of a known label on the gene probe and thus providing a direct measure of the content of Nitrosomonas strains in the sample of waste water or soil.
机译:用于测定废水和土壤中亚硝化单胞菌菌株的检测方法使用了一种基因探针,该基因探针由于其互补序列而仅与废水或土壤样本中的亚硝化单胞菌菌株的基因组部分杂交,而没有给出与其他细菌基因组部分的阳性杂交信号,需要通过基因探针上的已知标记定量测定杂交的核酸,从而直接测量废水样品中亚硝化单胞菌菌株的含量或土壤。

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