Fluorometric assay of total IgE level and reagents therefor

摘要

A method for identifying and quantifying total IgE levels in patient serum by conjugating the IgE in the serum with anti-lgE antibody adhering to an insoluble support, conjugating the serum IgE with an enzyme labeled anti-IgE antibody, contacting the enzyme label with a solution of a substrate which will yield a fluorescent product in the presence of the enzyme, and measuring the level of fluorescence in the solution. Special reagents and their manufacture are also disclosed.