RECOMBINANT PLASMID DNA PTHY314, ENCODING POLYPEPTIDE WITH PROPERTIES OF HUMAN @@@-THYMOSINE, RECOMBINANT PLASMID DNA PTHY12 - AN INTERMEDIATE PRODUCT FOR ITS CONSTRUCTION, AND THE STRAIN OF BACTERIA ESCHERICHIA COLI - A PRODUCER OF POLYPEPTIDE WITH PROPERTIES OF HUMAN @@@-THYMOSINE

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 biotechnology, especially genetic engineering. purpose u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 increased u0443u0440u043eu0432u043du00a0 fusion u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 with properties of u0442u0438u043cu043eu0437u0438u043du0430 ai. received u0440u0435u043au043eu043cu0431u0438 - u043du0430u0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u0430 u0440u0442u043du0443314, u043au043eu0434u0438u0440u0443u044eu0449u0430u00a0 peptide with properties of u0442u0438u043cu043eu0437u0438u043du044d and rights, strain of e.coll wsc in 5056 for synthesis of u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 with properties of u0442u0438u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u0435 u043eu0442u043du043eu0441u0438u0442u0441u00a0 to biotechnology, in particular to the genetic.u0438u043du0436u0435u043du0435u0440u0438u0438. u0442u0438u043cu043eu0437u0438u043d (one of the more than 30 pep - u0442u0438u0434u043eu0432 low u043cu043eu043b.u043c. (1000 - 15000). u0432u044bu0434u0435u043bu00a0u0435u043cu044bu0445 from thymus (u0442u0438u043cu0443u0441u0430), has a strong general u0438u043cu043cu0443u043du043eu0441u0442u0438u043cu0443u043bu0438u0440u0443 of activity.u0442u0438u043cu043eu0437u0438u043d "1 u0441u0438u043du0442u0435u0437u0438u0440u0443u0435u0442u0441u00a0 in the body in the form of a predecessor, u043cu043eu0437u0438u043du044du043eu0438 rights, and u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u0434 u043fu043bu0430u0437 - u043cu0438u0434u043du0430u00a0 dna u0440u0442u043du044312, u00a0u0432u043bu00a0u044eu0449u0430u00a0u0441u00a0 intermediate product u0434u043bu00a0 p u043eu043bu0443u0447u0435u043du0438u00a0 plasmid u0440u0442u043du0443314.u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u043fu043bu0430u0437u043cu0438u0434u0430 u0440u0442u043du044312 contains a synthetic gene u043fu043eu043bu0438u043fu0435u0433u043fu0438u0434u0430 with properties of u0442u0438u043cu043eu0437u0438u043du0430 and rights, consists of Hind ill / Cla i fragment of dna plasmids pCPG2 contained with a tandem u043fu0440u043eu043cu043eu0442u043eu0440u043eu0432 a2 and a3 u0440u0430u0447u0447u0435u0439 transcription of bacteriophage t7. a station for u0442u0440u0430u043du0441u043bu00a0u0446u0438u0438, genes / lac - u0442u0430u043cu0430u0437u044b and u0445u043bu043eu0440u044du043cu0444u0435u043du0438u043au043eu043bu044du0446u0435u0442u0438u043bu0442u0440u0430u043du0441u0444u0435u0440u0433 - u0437u044b and transcription terminator u0444u0430u0433u0430).Clal / Htnd ii fragment containing the s & m u0442u0435u0442u0438u0447u0435u0441u043au0438u0439 gene. encrypting c: mu: one peg, u0442u0438u0434u0442u0438u043cu043eu0437u0438u043du0430 s rights. u0440u0435u043au0441u043cu0441u0447 - u043cu0437u0433 - u043du00a0u00a0 u043fu043bu0430u0437u043cu0438u0434u043du0430u00a0 dna u0440u0442u043du0443u0437 4 contains the gene u043fu043eu043bu0438u043fu0435u043fu0442u0438u0434u0430 with properties of u0442u0438u0440u043eu0437u0438u043du0430 and rights, which u043au043eu043du0441u0442u0438u0442u0443u0442u0438u0432u043du043e u044du043au0441u043fu0440u0435u0441u0441u0438u0440u0443 - et in the cells of e. coli in the u0445u0438u043cu0435u0440u043du043eu0442 u043fu0441u043fu0443.u044d tumor necrosis factor alpha - u0442u0438u043cu043eu0437u0433u043d;.strain of escherichia coli SG20050 / pTHV314 provides the level of biosynthesis u0442u0438u043cu0443u0441u043du043e of u043fu0435u043fu0442u0438u0434u0430 u0442u0438u043cu043eu0437u0438u043du0430 and rights to 8 to 10 micrograms / ml, with a simplified technique;. it u0432u044bu0434u0435u043bu0435u043du0438u00a0. 3 boeing. s - lu. from which it u043eu0442u0449u0435u043fu043bu00a0u0435u0442u0441u00a0 and then u043fu043eu0434u0432u0435u0440u0433u0430u0435u0442u0441u00a0 n - u043au043eu043du0446u0435u0432u043eu043cu0443 u0430u0446u0435u0442u0438u043bu0438u0440u043eu0432u0430u043du0438u044e.n - u0434u0435u0437u0430u0446u0435u0442u0438u043bu0442u0438u043cu043eu0437u0438u043d sp has a set of biological activities u043du0430u0442u0438u043fu043du043e on u043fu0435u043fu0442u0438u0434u0430, in particular u0442u0438u043cu043eu0437u0438u043d s stimulates t cells, u0438u043du0434u0443u0446u0438u0440u0443u00a0 fusion u0438u043du0442u0435u0440u043fu0435u0439u043au0438u043du0430 - 2 and - interferon, u0434u0435u043bu0430u00a0 them effective against a wide u0440u00a0u0434u0430 pathologies, such as Ssl i and it