METHOD FOR EVALUATING ANTILYSOZYMIC ACTIVITY OF MENINGOCOCCUS STRAINS

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to medicine and medical microbiology. the purpose of u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 simplification method, u043fu043eu0438u0437u043eu0431u0440u0435u0442u0435u043du0438u0435 u043eu0442u043du043eu0441u0438u0442u0441u00a0 to the field of medicine and medical microbiology. lysozyme has the ability to u043fu043eu0434u0430u0432u043bu00a0u0442u044c growth of many bacteria. therefore, strains of microorganisms, which u0438u043du0433u0438u0431u0438 - u0440u0443u044eu0442 this enzyme have significant advantage in interspecies competition.they should survive and u0432u043du0443u0442u0440u0438u043au043bu0435u0442u043eu0447u043du043eu0439 localization. u043fu0435u0440u0435u0432u0430u0440u0438u0432u0430u043du0438u0435 u0430u043du0442u0438u043bu0438u0437u043eu0446u0438u043cu043du044bu0445 strains in u0444u0430u0433u043eu0446u0438u0442u0430u0445 is many times slower than cells with no activity in the u043eu0442u043du043eu0448u0435u0432u044bu0448u0435u043du0438u0435 sensitivity and usk praha u043eu0440u0435u043du0438u0435 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 activity. method u0437u0430u043au043bu044eu0447u0430u0435u0442u0441u00a0 mixing suspended u043cu044du043du0438u043du0433u0441u043au043eu043au043au043eu0432 studied strains with the concentration of 5.0 ~ 10.0 billion, microbial cells in 1 ml of saline u043bu0438u0437u043eu0446u0438u043cu0430.to them in u0433u0440u0437u0434u043fu0435u043du0442 concentration u043bu0438u0437u043eu0446u0438u043cu0430, u0438u043du043au0443u0431u0438u0440u043eu0432u0430u043du0438u0438 30 - 60 min the mixture, followed by the definition of residual u043bu0438u0437u043eu0446u0438u043cu0430 and u0437u0430u0441u0435u0432u043eu043c in holes u0433u0435u043bu044cu0431u0437u043au0442u0435u0440u0438u0430u043bu044cu043du043eu0439 p u043eu043bu0438u0430u043au0440u0438u043bu0430u043cu0438u0434u043du0435u0439 environment, which includes the t e s t - l b t i r i Micrococcus Lysodekticus. with the concentration gradient of enzyme u0433u043eu0442u0441u0435u00a0u0442 at 0.25. 05 and 1.0. method u043fu043eu0437u0432u043eu043bu00a0u0435u0442 with high ei u0441u043bu0435u043bu0435u043du044cu044e - mid units.the ability of strains to identify ing / u044cu0438u0440u043e - t u00a0 t b enzyme in u0443u0441u043bu043eu0432u0438u00a0u0445, close to u0432u043du0443u0442u0440u0438u043au043bu0435u0442u043eu0447u043du043eu043cu0443 u043fu0430u0440u0430u0437u0438u0442u0438u0440u043eu0437u0437u043au043du044cu04331 micro-organisms in 12 to 15 times to shorten the time of iss u043bu0435u0434u043eu0432u0430u043du0438u00a0 and so u0435u043bu044cu043du043e simplify and u0443u0434u0435u0448u0435u0432u0438u0442u044c method u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 u0437u0447u0442u0438 - u043bu0438u0437u043eu0446u0438u043cu0447u043eu0439 activity with..u0435u0436u0435u0432u044bu0434u0435u043bu0435u043cu043du044bu0445 strains of meningococcus. table 5. (l with the enzyme.in addition, microorganisms with these properties can u0432u043du0443u0442u0440u0438u043au043bu0435u0442u043eu0447u043du043eu043cu0443 reproduction. u043cu0435u043du0438u043du0433u043eu043au043eu043au043au0438 u043eu0442u043du043eu0441u0438u0442u0441u00a0 to u0432u043du0443u0442u0440u0438u043au043bu0435u0442u043eu0447u043du044bu043c parasites and have u0430u043du0442u043du043bu0438u0437u043e - u0446u0438u043cu043du0441u0439 activity (ala). a wide range of values of the ala is u0434u043bu00a0 different strains and clones u043fu043eu043fu0443u043bu00a0u0446u0438u0438.the presence of this factor u0440u0430u0441u0446u0435u043du0438u0432u0430u0435u0442u0441u00a0 as one of the possible targets u043fu0430u0442u043eu0433u0435u043du043du043e of microorganisms, which can be used in the epidemiological ha u0440u0430u043au0442u0435u0440u0438u0441u0442u0438u043au0435 strains. vj vj on on on