METHOD FOR IMMUNOFERMENTATIVE ANALYSIS OF THYROXIN CONTENT IN BLOOD SERUM

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to u0438u043cu043cu0443u043du043eu0445u0438u043cu0438u0438, more accurate ways of immune enzyme u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 biologically active substances using as a marker of firmin u0442u043du044bu0445 cells. purpose u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 simplification method by visual quantitative u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 u0442u0438u0440u043eu043au0441u0438u043du0430 in blood serum.the purpose of u0434u043eu0441u0442u0438u0433u0430u0435u0442u0441u00a0 so that activation of the porous u043du043eu0441u0438u0442u0435u043bu00a0 lead u0434u0438u044du0442u0438 - u043bu0430u0446u0435u0442u0430u043bu0435u043c p - u0430u0437u0438u0434u043eu0431u0435u043du0437u0430u043bu044cu0434u0435u0433u0438u0434u0430 followed by radiation with uv light, which light is j cm 2. u043fu043eu0441u043bu0435u0434u0443u044eu0449u0430u00a0 u043au0438u0441u043bu043eu0442u043du0430u00a0 treatment leads to the formation of surface u0440u0435u0430u043au0446u0438u043eu043du043du043e is capable of u0430u043bu044cu0434u0435u0433u0438u0434u043du044bu0445 groups.the number of u0430u043bu044cu0434u0435u0433u0438u0434u043du044bu0445 groups per unit area u043eu043fu0440u0435u0434u0435u043bu00a0u0435u0442u0441u00a0 u043eu0441u0432u0435u0449u0435u043du043du043eu0441u0442u044cu044e of site surface. in a subsequent processing u0430u043au0442u0438u0432u0438u0440u043eu0432u0430u043du043du043eu0439 surface with specific antibodies is u043au043eu0432u0430u043bu0435u043du0442u043du043eu0435 u0441u0432u00a0u0437u044bu0432u0430u043du0438u0435 through u0430u043cu0438u043du043eu0433u0440u0443u043fu043fu044b with u0430u043bu044cu0434u0435u0433u0438u0434u043du044bu043cu0438 gr u0443u043fu043fu0430u043cu0438 u043du043eu0441u0438u0442u0435u043bu00a0. u043fu043eu0432u0435u0440u0445u043du043eu0441u0442u043du0430u00a0 antigen density is proportional to the amount of u0430u043bu044cu0434u0435u0433u0438u0434u043du044bu0445 groups on the surface.u043du0438u0436u043du00a0u00a0 and u0432u0435u0440u0445u043du00a0u00a0 borders the illumination due to the need to u043eu043fu0440u0435u0434u0435u043bu00a0u0442u044c u0442u0438u0440u043eu043au0441u0438u043du0430 level in blood serum in a physiological range of con u0446u0435u043du0442u0440u0430u0446u0438u0439.when diving strips with u043au043eu0432u0430u043bu0435u043du0442u043du043e u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044bu043cu0438 antibodies in the analyte solution on the surface of the strips is competitive u0441u0432u00a0u0437u044bu0432u0430u043du0438u0435 the scarred and u043du0435u043cu0435u0447u0435u043du043eu0433u043e (sample) u0442u0438u0440u043eu043au0441u0438u043du0430 with u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044bu043cu0438 on strip antibodies.the number of the scarred u0442u0438u0440u043eu043au0441u0438u043du0430, u0441u0432u00a0u0437u0430u043du043du043eu0433u043e in immunization supplements, u043eu043fu0440u0435u0434u0435u043bu00a0u0435u0442u0441u00a0 concentration of test substance and surface concentration of u0438u043cu043cu043eu0431u0438u043bu0438u0437u043eu0432u0430u043du043du044b x antibodies.evaluation of u0434u043bu00a0 u0441u0432u00a0u0437u0430u043du043du043eu0439 u043fu0435u0440u043eu043au0441u0438u0434u0430u0437u043du043eu0439 marks on the recording medium (and hence, u0443u0440u043eu0432u043du00a0 u0442u0438u0440u043eu043au0441u0438u043du0430 in solution) strips is saline substrates, reza u043bu044cu0442u0430u0442u0435 u0444u0435u0440u043cu0435u043du0442u0430u0442u0438u0432u043du043eu0439 reaction u043eu0431u0440u0430u0437u0443u0435u0442u0441u00a0 dark blue insoluble product, u043eu0441u0430u0436u0434u0430u044eu0449u0438u0439u0441u00a0 on media in the field localization of enzyme tags.the intensity of the u043eu043au0440u0430u0448u0438u0432u0430u043du0438u00a0 strips is inversely proportional to the number of u043eu043fu0440u0435u0434u0435u043bu00a0u0435u043cu043eu0433u043e u0442u0438u0440u043eu043au0441u0438u043du0430 in solution and is indicative of the concentration of u043eu043fu0440u0435u0434u0435u043bu00a0u0435u043cu043eu0433u043e u0441u043eu0435u0434 u0438u043du0435u043du0438u00a0 in the original sample. the proposed method u043fu043eu0437u0432u043eu043bu00a0u0435u0442 visually quantitative analysis u0442u0438u0440u043eu043au0441u0438u043du0430 with sensitive, just as ordinary instrument methods ifa with u0444u043eu0442u043eu043cu0435u0442u0440u0438u0447u0435u0441u043au043eu0439 detection system. 2, table 2).(l (n3 on the 11th