STRAIN OF HYBRIDOUS CULTURED MAMMALIAN CELLS MUS MUSCULUS - A PRODUCER OF MONOCLONAL ANTIBODIES AGAINST ERYTHROCYTE ANTIGEN V OF CATTLE F-SYSTEM AND RELATED SPECIES

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 biotechnology and can be used in livestock u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 tribal groups blood cattle (cattle). purpose to obtain u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0 u00a0u0432u043bu00a0u0435u0442u0441u00a0 strain of hybrid cells, producing mono - u043au043bu043eu043du0430u043bu044cu043du044bu0435 antibodies (mon - ath), against the u044du0440u0438u0442 - u0440u043eu0446u0438u0442u0430u0440u043du043eu0433u043e antigen v f of the rdc. increasing the specificity and stability in storage.the strain is deposited with the number u0432u0441u043au043a (p) 188D and received the name of IC8 / 18 / 2, strain is produced by hybridization u0441u043fu043bu0435u043du043eu0446u0438u0442u043eu0432 mice Balb / c, u0438u043cu043cu0443u043du0438u0437u0438u0440u043eu0432u0430u043du043du043eu0439 u044du0440u0438u0442u0440u043eu0446u0438u0442u0430 mi rdc containing mr. antigen f - system of cattle, with mouse myeloma cells of sp 2 / 0 - ad 14. the cells Mus.musculus IC8 / 18 / 2 with the u0443u0441u043bu043eu0432u0438u00a0u0445 in vitro or in vivo studies in mice Balb / c u0434u043bu00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 u0441u0443u043fu0435u0440u043du0430u0442u0430u043du0442u043eu0432 u043au0443u043bu044cu0442u0443u0440u0430u043bu044cu043du043eu0439 environment, u0430u0441u0446u0438u0442u0438u0447u0435u0441u043au043eu0439 fluid or serum of mice, ods u0435u0440u0436u0430u0449u0438u0445 mon - al against antigen v f of the rdc, which u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0442 based reagent used u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 antigen v.mon at u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0442 an immunoglobulin class g3, u043du0430u043au0430u043fu043bu0438u0432u0430u044eu0442u0441u00a0 in u0430u0441u0446u0438u0442u0438u0447u0435u0441u043au043eu0439 fluid in u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0u0445, u043eu043fu0440u0435u0434u0435u043bu00a0u0435u043cu044bu0445 credits 1: 4000 - 1: 100000, don't reduce u0430u043au0442u0438u0432u043du043eu0441u0442 and for the storage of u0430u0441u0446u0438u0442u0438u0447u0435u0441u043au043eu0439 fluid within 9 u043cu0435u0441u00a0u0446u0435u0432 at (- 10) - (12) 1 & deg; c, with three times the freezing - u043eu0442u0442u0430u0438u0432u0430u043du0438u0438 and u043bu0438u043eu0444u0438u043bu0438u0437u0430 function.in addition to the main branch of the invention may find application in the scientific u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0u0445 and medical jurisprudence to / with 4 v | vj