method for thermoinduzierbaren gene expression in gram positive bacteria, bacillus subtilis staemme, plasmids containing such expression to induce and portable vector systems.

摘要

Thermo-inducible gene expression in Gram positive bacteria is optained through the insertion in the bacteria of two different kinds of plasmid containing respectively, on the first plasmid a promotor and a heterologous structural gene and on the second plasmid a repressor gene for the promotor located on the first plasmid and a temperature sensitive entity. The process allows the synthesis of different kinds of proteins through Gram positive bacteria induced by a temperature change.