Human mitochondrial NAD(P)-dependent malate enzyme - used to study formation of pyruvate from aminoacid(s) in tumour cells

摘要

Human mitochondrial NAD(P)+-dependent malate enzyme (I) and DNA (II; sequence of about 1900 bases reproduced) which encodes it are new. Also new are (1) recombinant DNA (IIa), contg. (II) or a fragment of it; (2) host organisms (esp. E. coli) transformed with (IIa); (3) recombinant (I), esp. in biologically active form (sequence of 584 amino acids reproduced); (4) monoclonal antibodies (MAb) against (I); (5) hybridomas which produce MAb. USE/ADVANTAGE - (I) catalyses conversion of malate to pyruvate. (I) and (II) (and modified versions of them) are useful for studying carbon metabolism in rapidly dividing cells, esp. pyruvate formation from amino acids. MAb are useful (a) to produce immuno-affinity materials for purificn. of (I) and (b) to determine (I)