Strains of pichia pastoris created by interlocus recombination

摘要

A process for the regeneration of a selectable phenotype at a high frequency by interlocus recombination, in yeast cultures transformed with recombinant DNA constructs comprised of a gene whose product complements the selectable phenotype to be regenerated is disclosed. A sequence of steps is involved including; suspending together in rich media mutant yeast strains, plating onto presporulation agar, replica plating onto other agar media suitable for selection and growth of diploid cells, plating on sporulation agar or alternatively dissecting the asci, digesting the cells, growing the spores on minimal media, and screening for the desired phenotype.