PHOSPHORIC ACID REMOVING REAGENT IN CLINICAL INSPECTION LIQUID AND HYDRAULYSIS OF STEROID CONJUGATED TREATED THEREBY

摘要

PURPOSE:To elevate the phosphoric acid removing effect in an inspection liquid, by arranging a phosphoric acid removing reagent composed of a first reagent in which MgCl2-NH4Cl and a second reagent of an ammonia solution. CONSTITUTION:A phosphoric acid removing reagent in a clinical inspection liquid is composed of the following first and second reagents: 2.4-3.3g of MgCl2 (5-7g for MgCl2.6H2O) and 10-13g of NH4Cl dissolved in a deioned solution per 50ml thereof and 25-28% of an ammonia solution. 100-300mul of the first reagent and 50-70mul are added to an inspection liquid controlled toward the alkali side from pH6.5 per 1ml thereof and after the agitation thereof, the mixture undergoes centrifugal operation and a conjugated hydrolytic enzyme is acted upon the supernatant. Thus, the use of the reagents produces a high effect of removing phosphoric acid in the inspection liquid while enabling the removal of phosphoric acid in the inspection liquid by a handy processing means.