OLIGONUCLEOTIDE FOR DETECTING BACTERIUM BELONGING TO GENUS CAMPYLOBACTER, METHOD FOR DETECTING BACTERIUM BELONGING TO GENUS CAMPYLOBACTER AND REAGENT KIT FOR DETECTION

摘要

PURPOSE: To obtain a new oligonucleotide useful for rapidly and simply carrying out the detection of a bacterium belonging to the genus Campylobacter and/or the identification of the bacterial species belonging to the genus Campylobacter. ;CONSTITUTION: The objective oligonucleotide has a nucleic acid sequence expressed by formulas I to XIV or a sequence complementary thereto. This oligonucleotide specific for a pathogenic bacterium belonging to the genus Campylobacter, especially Campylobacter.jejuni or Campylobacter.coli is expressed by formulas I to XII preferably formulas VII to X and XII. Various oligonucleotides are synthesized according to a phosphoramidite method by using a DNA synthesizer model 391 manufactured by Applied Biosystems, Inc. (ABI) and then purified by a reversed phase column of fast protein liquid chromatography(FPLC) manufactured by Pharmacia AB.;COPYRIGHT: (C)1993,JPO&Japio