Preparation manner and device of picking and treatment and minute vascular endotherium the adipose tissue

摘要

A device is provided, and a method, for collecting and processing tissue to produce an endothelial cell product having a vessel for rinsing, draining, digesting and isolating tissue. The vessel has a rinsing and digesting chamber for containing tissue during processing. An inlet in the rinsing and digesting chamber allows entry of rinsing solution and tissue from liposuction means. A drain chamber is in the vessel and is in fluid communication with the rinsing and digesting chamber. The drain chamber connects with a vacuum source. A screen in the vessel separates the rinsing and digesting chamber from the drain chamber to allow filtered transfer. An isolation chamber in the vessel is separated by another screen. A channel in fluid communication with the isolation chamber includes a pair of ports controlled by valve means to be selectively in fluid communication with the channel. After processing, the channel has a pellet of microvessel endothelial cells and the valve means may place the pellet of microvessel endothelial cells in fluid communication with the ports. The method includes providing the vessel, introducing tissue to be processed, orienting the vessel to screen the tissue, introducing an enzyme and agitating to digest the tissue, centrifuging the vessel to transfer the cells from the digested tissue, and isolating the cells for retrieval.