PRODUCTION OF BIOTINIZED CDNA LIBRARY AND SUBSTRACTION USING THE SAME

摘要

PURPOSE: To provide a production of cDNA library for easy and efficient subtraction using cDNA and a method for an easy subtraction using the method. ;CONSTITUTION: RNA extracted in a usual manner is annealed with poly (A) + RNA using 5'-biotin-labeled oligo dT (18 mer) as a primer and DNA is synthesized by a reverse transcriptase to obtain this biotinized cDNA library. The biotinized cDNA is hybridized with the mRNA of the cells to be subtracted, then ligated with polystyrene beads containing magnetizable substance bonded to streptavidin and the mRNA which has not been hybridized and remaining in the liquid phase is taken out to prepare the subtraction library.;COPYRIGHT: (C)1993,JPO&Japio