MALTOOLIGOSIDE DERIVATIVE, REAGENT FOR MEASURING ALPHA-AMYLASE ACTIVITY CONTAINING THE SAME AS ACTIVE INGREDIENT AND MEHTOD FOR MEASURING ALPHA-AMYLASE ACTIVITY USING THE SAME

摘要

PURPOSE: To obtain a new maltooligoside derivative, useful as a reagent for measuring the α-amylase activity and capable of efficiently and accurately measuring the a-amylase activity. ;CONSTITUTION: A maltooligoside derivative expressed by formula I [(n) is 3-5; R is aromatic coloring group; X is =CHCH₂N₃ or =C=CH₂; Y is H, hydrocarbon, etc.], e.g. 2-chloro-4-nitrophenyl-6⁵-azido-6⁵-deoxy-β-D-maltopentaoxide. The aforementioned compound is obtained by reacting a D-maltooligoside expressed by formula II with a carbonyl compound expressed by formula III (R¹⁶ is H, methoxy, etc.; R¹⁷ is methoxy or ethoxy), providing a compound expressed by formula IV, then successively subjecting the resultant compound to acylation, removal of alkoxymethylidene group and alkylsulfonylation, subsequently iodinating and azidating the resultant product, affording an acylmaltooligoside derivative expressed by formula V(R¹⁸ is acyl; W² is acyl, hydrocarbon, etc.) and then deacylating the aforementioned compound.;COPYRIGHT: (C)1993,JPO&Japio