PROCEDURE FOR OBTAINING A2 PHOSPHOLIPASE FROM THE SEA ANEMONE STAICHACTIS HELIANTHUS AND THE PRODUCT THEREBY OBTAINED.

摘要

Relates to the field of biochemistry, specifically the production of toxins plipeptidica nature with fosfolipasica activity.The Invention develops a procedure for the purification of phospholipase A2 from the total extracts of the AnemoneMarina Stoichactis Helianthus.The purification procedure consists in the combination of the method of preparation of the extracts the entire Body of the animal,The Chromatography on Sephadex g-50 Chromatography on cm Cellulose and 52 in specific conditionsWhat makes it possible to obtain the Enzyme with a satisfactory Performance and degree of purity.Phospholipases A2 have application in Research Related to the Study of biomembranes.In addition to the pharmacological Properties present may have a wide use in the field of biomedicine.In the field of Biotechnology are used as Enzyme Reactors in obtaining lisofosfatidos.The Purified phospholipase A2 with Helianthus Anemone Stoichactis manifests a High haemolytic activity directly, a feature uncommonThis is of interest because of their potential use in the preparation of immunotoxins for lysis of tumor cells. !