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YEAST PRODUCTION OF HEPATITIS B SURFACE ANTIGEN UNDER THE INFLUENCEOF A PROMOTER ISOLATED FROM PICHIA PASTORIS
YEAST PRODUCTION OF HEPATITIS B SURFACE ANTIGEN UNDER THE INFLUENCEOF A PROMOTER ISOLATED FROM PICHIA PASTORIS
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机译:分离自毕赤酵母的启动子对酵母菌乙型肝炎表面抗原的生产
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摘要
Novel DNA constructs comprising regulatory regions plus the structural coding region for hepatitis B surface antigen (HBsAg) are disclosed. The regulatory regions employed are responsive to methanol, non-catabolite repressing carbon sources and catabolite repressing carbon sources followed by carbon source starvation. The novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used to transform suitable hosts and ultimately used for the production and isolation of hepatitis B surface antigen in high yields.
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