BAKTERIJU ESCHERICHIA COLI KAMIENAS-RESTRIKTAZES ECO 130 1 PRODUCENTAS

摘要

the invention u043eu0442u043du043eu0441u0438u0442u0441u00a0 to biotechnology, to u0432u044bu00a0u0432u043bu0435u043du0438u044e microorganism capable of producing the site - specific u044du043du0434u043eu043du0443u043au043bu0435u0430u0437u0443 - u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u0443. u0440u0435u0441u0442u0440u0438u043au0442u0430u0437u0430 can be used u0434u043bu00a0 u0438u0441u0441u043bu0435u0434u043eu0432u0430u043du0438u00a0 primary structure of dna, and genetic engineering.the cultivation of u043fu0440u043eu0434u0443u0446u0435u043du0442u0430 Kscherichia coli wsc r - 4498 (UFL 130 u043fu0440u043eu0432u043eu0434u00a0u0442 in u0443u0441u043bu043eu0432u0438u00a0u0445 aeration in 37 & deg; c on the nutritional environment containing, g / l: u043fu0435u043fu0442u043eu043d 1; yeast extract; 5 control valve 10. ph 7.0 7.2 to the optical density of the suspension of cells u0434u043eu0441u0442u0438u0436u0435u043du0438u00a0 3.0 3.7. e. purification of enzyme u043fu0440u043eu0432u043eu0434u00a0u0442 u0445u0440u043eu043cu043b - u0442u043eu0433u0440u0430u0444u0438u0447u0435u0441u043au0438u043c by. solution the enzyme u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 40000 units. 1 g of biomass. 1, table 2). r