MODIFICATION OF THE DNA SEQUENCE BETWEEN THE SHINE-DALGARNO SEQUENCE AND THE START CODON OF THE TRP OPERON TO INCREASE PROTEIN EXPRESSION

摘要

- 1 - HOE 85/F 072Abstract of the Disclosure:When, in the trp operon of E. coli, the adenosine immedi-ately upstream of the ATG start codon is replaced bycytidine, and guanosine is connected downstream of thestart codon ATG, then an Nco I cleavage site which permitscloning is obtained. The operon thus modified results invery strong expression of the gene downstream of the startcodon. In addition, it is possible for the formation of.beta.-lactamase to be increased, which results in increasedampicillin resistance, which can be utilized for selec-tion.