A CLONING VEHICLE FOR CLONING A LARGE FRAGMENT OF DNA AND A VACCINE COMPRISING 987P FIMBRIAL PROTEIN

摘要

A new method of cloning large fragments of DNA by inserting the fragment into a cloning vehicle which also contains a high copy plasmid and a partition locus. The process of the invention is particularly useful for producing 987P fimbrial proteins by transformed E. coli, for incorporation into vaccines for the prevention and treatment of scours in piglets. The process is also useful as a tool in the creation of DNA libraries for eukaryotes.