METHOD OF DEHYDRAGENASE ACTIVITIES DETERMINATION IN PEACH LEAVES

摘要

Usage: region biochemical genetics methods for determining isoenzymes - digedrogenaz in leaves peach plant. The inventive method comprises applying a preparation of enzymes by 20% polyacrylamide gel and used as a buffer system compositions - Tris - 215 g EDTA - 18 g, NzVOz - 110 g, HCG to 2.5 L at pH 8.3, diluted with water in a ratio of 1: 7 for the electrode buffer and 1: 3 for the gel buffer. As coloring solutions using Tris HCI buffer pH 8.5 in an amount of 7 ml plus 28 ml H20, NAD -2 mg MTT - 2 mg, MBF - 5 mg plus 3 ml of alcohol for identification alkogolde- hydrogenase. To identify laktatde- hydrogenase use composition: 0.5 M phosphate buffer pH 7.4 in an amount of 7 ml plus 28 ml of H20, 550 ml of sodium lactate, MBF - 2 mg MTT 15 mg and 15 mg. 2 yl. The disadvantage of this method is that it allows the define a relatively small portion of isozyme system in plant peach. Known is a method for determining dehydrogenase dehydro in leaves peach plants (prototype), consisting of a preparation of partially purified enzyme preparation, applying it to gel electrophoresis followed by incubation in medium containing a buffer solution, VJ about 00 about 00 w (l