Cloning syringe and method for separating community using this cloning syringe

摘要

The present invention relates to a cloning syringe apparatus filled with a semi-solid material such as white petrolatum or lanolin or grease, and a method of using the same.;In the field of genetic engineering, in order to know the mass production of a specific gene product, or the characteristics of a genetic factor, transfection of a specific gene into eukaryotic cells involves the step of separating overexpressed eukaryotic cells only with specific genes, The greater the number of populations emerging from the spectrum, the more likely it is to obtain cells that overexpress proteins of a particular gene.;According to the present invention, a sterilized white petrolatum or the like is filled into a cloning syringe, and a proper amount of filler material is closely adhered to the periphery of the community exactly according to the size of the community, so that more communities can be separated in a short time, This is convenient and therefore makes it possible to achieve high success in the transfection and selection process of the specific gene overexpressing cells.