STRAIN OF HYBRID CULTURED CELLS MUS MUSCULUS L USED FOR PREPARING OF MONOCLONAL ANTIBODIES FOR @@@-DEPENDENT DNA-ASE IN RAT LIVER CHROMATIN

摘要

Use: biotechnology, molecular enzymology. SUMMARY OF THE INVENTION Preparation of the strain cultured hybrid cells Mus musculus L., producing monoclonal antibodies (mAb) to Mn -dependent DNase chromatin rat liver. The strain obtained gidridiza- tion splenocytes mice Balb / c cells and myeloma NS / 1 - 4.1 Ag. ICA titer in the culture fluid, determined by ELISA was 1: 8, ascitic - 1:10 .MKA bind Mn2 + -dependent DNase, and they are capable of inhibiting nuclease activity of the enzyme. Table 2.