METHOD FOR PRODUCING DEOXYRIBONUCLEIC ACID CODING SALMON GROWTH HORMONE: METHOD OF CONSTRUCTING INTERMEDIATE RECOMBINATION PLASMIDE DNA INCLUDING DNA FRAGMENT CODING SALMON GROWTH HORMONE FOR PRODUCING P S G H1, P S G H3, P S B H9, P S G H10, P S G H14 AND P S G H17 PLASMIDES: METHOD OF PRODUCING RECOMBINATION PLASMI

摘要

The invention relates to genetic engineering and relates to the preparation of cDNA coding for a fish growth hormone produced by recombinant DNA plasmid encoding the hormone, as well as a method for preparing fish growth hormone polypeptide using the microorganism. Summary of the Invention; messenger RNA extracted from the pituitary gland of salmon, after which the synthesized DNA complementary to the mRNA (cDNA). A DNA sample corresponding to the amino acid sequence. NIKON tsa salmon growth hormone, and then cloned salmon growth hormone gene by selecting cDNA which hybridizes with the DNA probe, and determining the base sequence of DNA. Describes expression and accumulation in the medium of salmon growth hormone by culturing a microorganism containing a recombinant DNA nantnuyuplazmidnuyu into which is introduced a cDNA encoding the salmon growth hormone. 4 h. n. f-ly. J