METHOD OF EXPRESSION OF DACS/DAOCS ACTIVITY IN ESCHERICHIA COLI CELLS

摘要

use: / u0435u043du0435u0442u0438u0447 u0435u0441u043au0430u00a0 u0438u043du0436u0435u043du0435u0440u0438u00a0, u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du0430u00a0 u0442u0435u0445u043du043eu043bu043eu0433u0438u00a0 u043fu043eu043bu0443u0447u0435u043du0438u00a0 enzymes. the essence of the u0438u0437u043eu0431u0440u0435u0442u0435u043du0438u00a0: way of expression DACS / DAOCS activity in e. coli cells involves the concept of e. coli k12 strain u0442u0440u0430u043du0441u0444u043eu0440u043cu0430 Im109 u043fu043bu0430u0437u043cu0438u0434u043eu0439, followed by the selection of clones, ek with the u043fu0440u0435u0441u0441u0438u0440u0443u044eu0449u0438u0445 this activity. 12).