A process for the measurement of hydrogen peroxide, or for the measurement of a peroxide free mach ends of enzyme or biochemical substrate.

摘要

To an aqueous sample containing hydrogen peroxide there are added (a) an aromatic hydroxy compound (e.g. a phenol or naphthol which may have substituents), (b) a diamine compound (e.g. an aniline compound, e.g. a p-amino aniline), and (c) an oxidase enzyme (peroxidase or catalase), and after mixing and standing for 5 minutes to form a quinomic dye the depth of color of the dye in solution is measured and compared to a control, to measure the amount of the H₂0₂.;An enzyme (e.g. uricase) and its substrate substance (e.g. uric acid) from which H₂0₂ is liberated can be mixed to form the H₂0₂ sample and from the dye measurement the amount of either the enzyme or its substrate can be calculated.;The method is reproducible and rapid and can be used in an automatic analyzer.