New DNA probe for Staphylococcus haemolyticus identification - comprising 900 to 1500 base pair fragment from Pst I cleaved S. haemolyticus, pref. used in dot blot

摘要

A DNA probe for the identification of Staphylococcus (S.) haemolyticus derived from S. haemolyticus DNA cleaved with PsrI is claimed. Also claimed is a method, e.g. a dot blot, for S. haemolyticus determn. in a DNA sample using the probe and kit for performing the method contg. the probe, buffer and auxiliary agents. USE/ADVANTAGE - S. haemolyticus comprises 10% of those coagulase-negative S. which are prim. pathogens causing e.g. infections of implants, endophthalmitis and endocarditis. Therefore, it is necessary to clearly identify S. haemolyticus. Prior art methods used biochemical methods for the distinction of different S. strain. However, a clear distinction between related strains is easy using the new proberef