DNA constructs containing a kluyveromyces amp;agr; factor leader sequence for directing secretion of heterologous polypeptides

摘要

DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces &agr;-factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis &agr;-factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.