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Cloning and expressing restriction endonucleases and modification methylases from xanthomonas
Cloning and expressing restriction endonucleases and modification methylases from xanthomonas
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机译:黄单胞菌的克隆和表达限制性内切酶和修饰甲基化酶
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摘要
The present invention is directed to recombinant hosts which contain and express XhoI and XhoII Type II restriction endonuclease and/or M.XhoI and M.XhoII modification methylase genes. The present invention is also directed to vectors or DNA molecules which contain these genes, and to methods of producing these enzymes. One source of these enzymes is Xanthomonas campestris pv. holcicola, although other microorganisms may be used to isolate the restriction endonuclease isoschizomers and modification methylase isoschizomers of this invention.
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