Particle-mediated transformation of perennial fruit plants capable of adventitious budding on micropropagated tissue

摘要

Cranberry has been genetically engineered using a particle mediated transformation procedure. The tissues transformed were nodes from which adventitious buds were induced. A flooding technique using the antibiotic kanamycin was used to select for transformants while limiting escapes. A gene for the toxin from Bacillus thuringiensis was introduced into the transgenic cranberry to confer insect toxicity.