L-fucose dehydrogenase, a process for production thereof, quantitative assay for L-fucose using said enzyme and a kit for quantitative assay

摘要

L-Fucose dehydrogenase having the following Physicochemical properties (1) and (2):PP(1) Action and substrate specificity PPIt withdraws hydrogen from L-fucose and converts into L- fuconolactone and at the same time, reduces coenzyme NADP.sup.+ to NADPH. PP(2) pH and stable pH rangeP PWhen Tris- imidazole-sodium acetate buffer solution is used, the optimum pH is in a range of 9.0 to 10.0 and the stable pH range is between 8.0 and 10.5. PPThe L-fucose dehydrogenase utilizing NADP.sup.+ as coenzyme can be obtained by culturing L-fucose dehydrogenase-producing strain belonging to the genus Pseudomonas in a medium and collecting formed L- fucose dehydrogenase from the culture. PPL-Fucose can be quantitatively determined by reacting a sample containing L-fucose with L- fucose dehydrogenase requiring NADP. sup.+ as coenzyme and assaying NADPH produced.