The new plasmid and its construction manner and the new microbe null which contains that plasmid

摘要

PURPOSE:To produce a useful transformed bacterium, by forming a plasmid integrated with a deoxyribonucleic acid fragment carrying genetic information participating in secretory production of alkali CGTase of a bacterium belonging to the genus Bacillus at a restriction site. CONSTITUTION:A DNA fragment providing secretory production ability of alkali cyclomaltodextrin glucanotransferase (CGTase for short) of a bacterium belonging to the genus Bacillus is prepared by using a restriction enzyme. Vector DNA of plasmid is prepared by using a restriction enzyme which does not inhibit genetic information participating in secretory production of alkali CGTase of the DNA fragment. Further, the restriction site of the vector DNA of plasmid is removed with the above-mentioned DNA fragment and the recombinant plasmid is extracted to give a plasmid.