Hapten derivative, its manufacturing method, hapten / protein-conjugate, its manufacturing method, antibody manufacturing method and labeling enzyme for immunoassay

摘要

Hapten derivatives of the general formula (I) …IMAGE… in which Hap is the radical formed by elimination of an oxo group from a hapten with keto or aldehyde groups, and R1 and R2 are identical or different and are a lower alkyl group with 1 to 7, in particular 1 to 3, carbon atoms, and one radical R1 or R2 can also be a hydrogen atom, as well as the hapten-protein conjugates formed from these hapten derivatives (I), of the formula (III) …IMAGE… in which E-NH- is the radical of a protein bonded via an epsilon -amino group of a lysine residue, are described. …??The hapten-protein conjugates of the formula (III) are very suitable for use for the immunisation of organisms suitable for antibody production and as labelling enzyme in immunoassays.