BRAIN SODIUM-URINATING PEPTIDE OF MOUSE

摘要

PURPOSE: To provide a novel polypeptide useful for detecting and measuring a brain sodium-urinating peptide in a specimen and further useful as various reagents. ;CONSTITUTION: The polypeptide of the formula comprising 45 amino acids originated from a mouse. The polypeptide is obtained by screening the genom DNA library of the mouse with a DNA fragment coding the brain sodium- urinating peptide (BNP) of a rat as a probe, inserting the obtained DNA fragment coding the BNP of the mouse into a plasmid such as human interferon γ-fused glucagon-expressing plasmid, etc., transforming a host cell such as Escherichia coli K12 strain C600 with the architected expression vector, and subsequently culturing the transformant.;COPYRIGHT: (C)1994,JPO&Japio