MEGAKARYOCYTE DIFFERENTIATION AND PROLIFERATION FACTOR AND ITS PRODUCTION

摘要

PURPOSE: To provide the subject new proliferation factor obtained from a culture liquid prepared by culturing mouse spleen cell in the presence of a mitogen, effective in selectively differentiating and proliferating megakaryocyte, free from proliferation activity to other cells such as leukocyte and useful for the treatment of thrombocytopenia, etc. ;CONSTITUTION: A mouse-originated spleen cell is cultured in a medium such as RPM11640 medium in the presence of pokeweed-mitogen, the culture liquid is centrifuged, the supernatant is collected and incorporated with ammonium sulfate and the formed precipitate is collected. The precipitate is dissolved in 0.14M Nacl solution containing 10mM phosphate buffer solution and dialyzed with the solution to remove the ammonium sulfate. The dialyzed liquid is adsorbed to a DEAE sepharose column, an adsorbed fraction eluted with 0.5M Nacl aqueous solution containing 10mM phosphate buffer solution is collected and a protein component is separated from the fraction to obtain the objective megakaryocyte differentiation and proliferation factor inactivated by protease treatment and enabling selective differentiation and proliferation of megakaryocyte.;COPYRIGHT: (C)1994,JPO&Japio