METHOD FOR TESTING CYTOTOXICITY WITH CULTURED CELL

摘要

PURPOSE: To evaluate the toxicity of a specimen against skin tissues by separately culturing fibroblasts and epidermal cells in a matrix produced by filling a collagen gel in a collagen sponge, fixing the matrix on the interface between a medium and air and subsequently testing a specimen on the fixed matrix. ;CONSTITUTION: The evaluation of the cytotoxicity is performed by treating collagen with an enzyme to produce atherocollagen reduced in antigenicity, preparing the aqueous solution of the atherocollagen, adjusting the pH of the aqueous solution so as to increase the viscosity of the aqueous solution, homogenizing the aqueous solution into a creamy solution, pouring the creamy solution in a container whose bottom surface has preliminarily been coated with an aqueous solution of atherocollagen and then dried with air to form a thin coating film, gelating the poured creamy solution in the foam-containing state, vacuum-drying the gelation product to produce a collagen sponge, filling a collagen gel in the produced collagen sponge to produce a matrix, separately culturing fibroblasts and epidermal cells in the matrix, fixing the culture matrix to the interface between a medium and air, putting a specimen on the culture matrix and, after a constant time, counting the number of the alive cells.;COPYRIGHT: (C)1993,JPO&Japio