PROZONE CRITERION AND ANALYSIS METHOD IN ANTIGEN ANTIBODY REACTION

摘要

PURPOSE: To determine whether re-inspection is needed or not and the measurement conditions on re-inspection by performing prozone criterion easily by preventing wasting of an expensive reagent. ;CONSTITUTION: By obtaining a measurement ratio Vre at the 2 wavelengths at a first time immediate after initiation of reaction for a standard sample reaction liquid for creating a calibration curse and a measurement value ration Vre at 2 wavelengths at a second time after the reaction fully progresses, a regression straight line Yrc=a.Xre+b is obtained from a relationship of Vrs versus Vre. Vers out of a measurement ratio Vsrs at 2 wavelengths at a first time for a specimen reaction liquid and a measurement value ratio Vers at 2 wavelengths at a second time is substituted in Xre of the regression equation to obtain Yrc and it is compared with Vsrs, thus determining a prozone in the case of Yrc-αVsrs (α is an allowable value). In the case of the prozone phenomenon, a deviation rate (Yrc-Vsrs)/Yrc is calculated and re-inspection is performed under predetermined analysis conditions according to the value.;COPYRIGHT: (C)1994,JPO&Japio