Process for the preparation of recombinant gamma interferons having enhanced stability, and the vector and the DNA used in the method

摘要

Provided are methods for the production of mixtures of interferon (IFN)- gamma polypeptides. The methods employ a single species of IFN- gamma DNA having codons for Met-Gln and for residues 2-143 of the mature human IFN- gamma amino acid sequence. Expression of such DNA in a suitable recombinant host cell affords a mixture of polypeptides having N-terminal Met-Gln, H-Gln, or H-pyroglutamate residues and C-termini at Arg139, Ser142, or Gln143 of the mature IFN- gamma sequence. The polypeptides so produced exhibit the antiviral and antiproliferative activities as well as the acid lability characteristic of native human IFN- gamma .