USE FO TRANS-SIALIDASE AND SIALYLTRANSFERASE FOR SYNTHESIS OF SIALYL 2 3GALACTOSIDES

摘要

A single vessel cyclic synthesis process for preparation of a sialyl. alpha.2→3&bgr;galactoside is disclosed. In accordance with this process, a sialyltransferase acceptor is sialylated in an aqueous reaction medium by an &agr;(2,3)sialyl transferase and CMP-sialic acid to form a sialyl donor substrate and CMP. In the presence of the trans- sialidase of Trypanosoma crusi, that sialyl donor substrate provides a sialyl group for a trans-sialidase acceptor, thereby preparing the sialyl. alpha.2→3&bgr;galactoside. The &agr;(2,3)sialyltransferase acceptor is reformed upon trans-sialidation of the latter acceptor, and the sialyl donor substrate is reformed using the &agr;(2,3) sialyltransferase and a CMP-sialic acid recycling system that combines CMP with sialic acid that is also present in the vessel. The K.sub.m /V. sub.max value for the &agr; (2,3)sialyltransferase acceptor is less than one-tenth the value of K.sub. m /V.sub.max of the trans-sialidase acceptor for the &agr;(2,3) sialyltransferase.