Recombinant dna vectors encoding a 4'-0-isovaleryl acylase derived from a carbomycin biosynthetic gene, designated care, for use in streptomyces and other organisms

摘要

The carE gene of Streptomyces thermotolerans has been isolated and used to construct recombinant DNA expression vectors. The carE gene encodes 4"-O-isovaleryl acylase activity important in the biosynthesis of a number of useful antibiotics. The carE gene can be used not only to construct recombinant cells with an increased ability to produce the acylase enzyme but also to construct recombinant cells with the ability to produce novel antibiotic compounds.