METHOD FOR THE PRODUCTION OF NEUROTROPHIC AGEBTS AND METHOD FOR CLEANING THE NEUTROTROPHIC AGRNT OF THE SCIATIC NERVE CILLIA

摘要

1. Method of producing neurotrophic CNTF factor using recombinant DNA method, characterised in that (a) DNA sequence is prepared containing human or animal nucleic acid with formula depicted in figures 11 and 12, capable of directing host cell to produce a protein demonstrating CNTF activity, (b) Cloning the DNA sequence into a vector capable of being transferred into and replicated in a host cell, such vector containing operational elements needed to express the DNA sequence, (c) transferring the vector containing the synthetic DNA sequence and operational elements into a host cell capable of expressing the DNA encoding CNTF, (d) Culturing the host cells under conditions appropriate for amplification of the vector and expression of CNTF, (e) harvesting the CNTF; and (f) permitting the CNTF to assume an active tertiary structure whereby it demonstrates CNTF activity.IMAGE