PROCEDURE FOR THE ISOLATION OF MUTANTS, MUTED GENES, AS WELL AS THE CORRESPONDING WILD TYPE GENES.

摘要

MUTANTS CAN BE OBTAINED VERY EASILY BECAUSE THE TOTALITY OF THE DNA OF A BRANCH TO MUTE CAN BE ISOLATED, DIVIDED INTO SMALL FRAGMENT FRAGMENTS, INTEGRATED IN A WIDE RANGE OF PLATFORM, HIGH-RISE, HIGH-RANGE IT IS TRANSFORMED INTO STARTING BRANCH, THROUGH THE SELECTION BETWEEN THE MARKERS, THE TRANSFORMERS ARE SELECTED, THROUGH THE ELEVATION OF THE TEMPERATURE, ABOVE THE THRESHOLD OF THE VALUE OF THE TEMPERATURE-SENSITIVE PLASMIDS, THE PLASIDS ARE ELIMINATED , AND THROUGH THE RENEWED SELECTION OF THE MARKERS, THE MUTANTS ARE SELECTED. SINCE IN THIS WAY THE MUTANT GENE IS MARKED, IT CAN BE EASILY ISOLATED, AND THROUGH HYBRIDIZATION, FROM A WILD TYPE GENE BANK, NON-MUTANT GENES CAN BE ISOLATED.