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METHOD OF SELECTING GENETICALLY SUPERIOR SHRIMP

机译:遗传上优良虾的选择方法

摘要

A method for the selection of shrimp having a genetically-transmitted favorable growth characteristic for improved aquacultured shrimp production. A genetic marker for the favorable characteristic is prepared by isolating Penaeus shrimp nuclear DNA by extraction with chloroform in the presence of cetyl trimethyl ammonium bromide, digesting the DNA with one or more restriction enzymes, and identifying characteristic restriction fragments. The marker is hybridized to a nuclear nucleic acid molecule isolated from Penaeus shrimp for the selection of shrimp having the desired characteristic. The marker can be a labelled probe or a primer for amplification and subsequent detection of a gene that encodes a protein promoting the desired growth characteristic. The selected shrimp can be used to produce a high quality, genetically superior seedstock or larvae useful for the economic production of aquacultured shrimp.
机译:一种选择虾的方法,该虾具有遗传传递的有利生长特性,以改善水产养殖虾的产量。通过在十六烷基三甲基溴化铵存在下用氯仿萃取分离对虾虾核DNA,用一种或多种限制酶消化DNA,并鉴定特征性限制性片段,来制备具有良好特性的遗传标记。将标记物与从对虾分离的核核酸分子杂交,以选择具有所需特征的虾。标记物可以是标记的探针或用于扩增和随后检测编码促进所需生长特性的蛋白质的基因的引物。所选虾可用于生产高质量,遗传上优越的种或幼虫,可用于经济养殖虾。

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