LOW C0t DNA AS DNA FINGERPRINTING PROBE, AND METHOD OF MANUFACTURE

摘要

The present invention comprises both a for use in DNA fingerprinting analysis, and processes for making the probe and for using the probe. The probe comprises a quantity of low C0t DNA which has been isolated from a reference organism wherein the low C0t DNA has a plurality of repetitive DNA sequence types. The plurality of repetitive sequence types is at least two to all of the repetitive low C0t sequences. When used in the process of the present invention, the low C0t DNA multiple sequence probe enables the identification or exclusion of the subject organism. The process for producing the probe useful herein comprises the steps of isolating, shearing and denaturing a quantity of DNA from a reference organism, renaturing the DNA to an intermediate C0t, separating for further use the double stranded portions of the repetitive sequence low C0t renatured DNA from the nonrepetitive sequence DNA, and labeling the low C0t DNA.