Improved promoter, vector having the improved promoter, plasmid having the vector, and E.coli transformed with the plasmid

摘要

An improved promoter of E. coli, in which(a) the -35 region in the promoter shows the following base sequence wherein the XY base pair represents a base pair of either TA, GC, AT or CG,and/orthe -10 region in the promoter shows the follow­ing base sequence(b) an intermediate region between the -35 region and the -10 region in the promoter consists of 15 to 17 base pairs and shows such a base sequence in which the proportion of base pairs CG and GC in the entire base pairs in this region is at least 60 % and a vector having the improved promoter.