Method for the production of recombinant polypeptides bearing epitopes from different hiv isolates, and their uses as immunogens and in the detection of antibodies against hiv

摘要

The present invention provides recombinant polypeptides bearing selected epitopes from Human Immunodeficiency Virus (HIV) isolates.;The technical aim is to design and obtain, by means of chemical synthesis, genes encoding for a polypeptide composed not only by different HIV epitopes but also including epitopes corresponding to different HIV isolates. Said polypeptides correspond to the general formulae:;Where:I. X1 to Xn represent fragments or viral proteins capable of inducing neutralizing antibodies or peptidic fragments capable of stimulating the proliferation and activation of helper T lymphocytes.II. L represents spacer sequence or "linkers" located between two X regions.;Said polypeptides are capable of inducing neutralizing antibodies against HIV. Said polypeptides or antibodies generated against it could be useful for the diagnosis, treatment or prevention of AIDS.